Shot back-to-back with Anacondas: Trail of Blood (2009).
The mutant anaconda has the skills of spitting acid, more resistant skin, high fertility rate and a sting in the tail.
In several posters of the film the snake shown has the appearance of a python instead of a green anaconda that is the true antagonist of the franchise.
The baby anaconda picked up by Murdoch's assistant at the end of the movie is not a true anaconda, instead another species of boa.
It is the only film of the franchise that does not take place in a forest, the first film took place in the Amazon forest, the second in the tropical forest of Borneo, this third part takes place in a farming community in Romania, in the sequel again the scenario is a forest: a Romanian forest.